このコースについて
4.7
134件の評価
31件のレビュー

100%オンライン

自分のスケジュールですぐに学習を始めてください。

柔軟性のある期限

スケジュールに従って期限をリセットします。

中級レベル

約13時間で修了

推奨:5 weeks, 2-3 hours/week...

英語

字幕:英語

習得するスキル

BiologySample PreparationSample CollectionCell Biology

100%オンライン

自分のスケジュールですぐに学習を始めてください。

柔軟性のある期限

スケジュールに従って期限をリセットします。

中級レベル

約13時間で修了

推奨:5 weeks, 2-3 hours/week...

英語

字幕:英語

シラバス - 本コースの学習内容

1
2時間で修了

Introduction to the Course and the Field of Extracellular Vesicles

In this module, we will introduce the course and the field of extracellular vesicles. We will discuss the nomenclature and the history of extracellular vesicles as well as the organisms and places that have been shown to release/contain extracellular vesicles. ...
4件のビデオ (合計69分), 1 reading, 1 quiz
4件のビデオ
Introduction to the Course11 分
The Origin of EVs throughout the Phylogenetic Tree11 分
History of Exosomes and Extracellular Vesicles32 分
1件の学習用教材
Syllabus10 分
1の練習問題
Introduction to Extracellular Vesicles18 分
2
3時間で修了

Biogenesis, Cargo and Uptake of Extracellular Vesicles

In this module, we will focus on the biogenesis and release of extracellular vesicles as well as the uptake mechanisms of extracellular vesicles when they are encountered by a recipient cell. This module also covers the different RNA, protein and lipid types present in extracellular vesicles as well as a brief overview what the functions of these molecules are. Furthermore, the techniques that are commonly used to detect these molecules as well as databases that can be useful to use when analyzing the cargo of extracellular vesicles will be highlighted....
6件のビデオ (合計142分), 1 quiz
6件のビデオ
Mechanisms of Extracellular Vesicle Uptake - Part 122 分
Mechanisms of Extracellular Vesicle Uptake - Part 223 分
The Protein Content of Extracellular Vesicles23 分
The RNA Content of Extracellular Vesicles35 分
The Lipid Content of Extracellular Vesicles18 分
1の練習問題
Biogenesis, Cargo and Uptake of Extracellular Vesicles30 分
3
2時間で修了

Collection and Processing of Cell Culture Media and Body Fluids Prior to Isolation of Extracellular Vesicles

In this module, we will focus on the collection and processing of cell culture media and body fluids prior to isolation of extracellular vesicles. We will discuss some of the considerations and guidelines that are important during the collection of staring material and isolation of extracellular vesicles. This module will help you with the different choices, such as anticoagulants, collection time points and protein inhibitors, which are important for the outcome when working with a particular body fluid compared to conditional media or other body fluids. This module will also illustrate some example of studies on extracellular vesicles from body fluid such as blood, urine, breast milk and cerebrospinal fluid and why it is of interest to analyse extracellular vesicles from these body fluids. ...
5件のビデオ (合計79分), 1 quiz
5件のビデオ
Blood Plasma and Serum15 分
Breast Milk8 分
Urine25 分
Cerebrospinal Fluid15 分
1の練習問題
Collection and Processing of Cell Culture Media and Body Fluids Prior to Isolation of Extracellular Vesicles36 分
4
2時間で修了

Methods for Isolating Extracellular Vesicles

This module highlights some of the different methods used to isolate extracellular vesicles. Here the basic concepts for methods such as differential ultracentrifugation, density gradient, size exclusion and kit-based precipitation will be covered and some guidelines will be presented. Furthermore, this module will cover how the techniques are used in the field of extracellular vesicles as well as their limitations and benefits....
6件のビデオ (合計77分), 1 quiz
6件のビデオ
Differential Ultracentrifugation - Part 216 分
Density Gradient12 分
Size Exclusion Chromatography14 分
Kit-based Precipitation12 分
Summary of Isolation Methods for Extracellular Vesicles6 分
1の練習問題
Methods for Isolating Extracellular Vesicles22 分
4.7
31件のレビューChevron Right

50%

コース終了後に新しいキャリアをスタートした

人気のレビュー

by MHJan 11th 2019

Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.

by MVJan 31st 2018

The only problem I faced, has to do with the subtitles. They have lots of mismatches and mistakes. Nevertheless, the MOOC is undoubtedly great and pretty educational.

講師

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Cecilia Lasser

Researcher
Krefting Research Centre, University of Gothenburg

カリフォルニア大学アーバイン校(University of California, Irvine)について

Since 1965, the University of California, Irvine has combined the strengths of a major research university with the bounty of an incomparable Southern California location. UCI’s unyielding commitment to rigorous academics, cutting-edge research, and leadership and character development makes the campus a driving force for innovation and discovery that serves our local, national and global communities in many ways....

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